Error with Link — Application Pool

I have this site

http://www.ableslayer.com/portfolio/

and when you visit it, it lands in the 404 page. I uploaded that from my localhost to where it is running very well

when you visit this page

http://www.ableslayer.com/portfolio/index.php

the home will be viewed correctly but when you click on the links an error appears

The specified request cannot be executed from current Application Pool

what suppose to be wrong with this? please help me. thanks