This plugin allows you to create multiple, dynamic widget areas, and assign those widget areas to sidebar locations within the Genesis Framework on a
No. You can only assign them to the primary and secondary sidebars, using the plugin.
However, once a widget area has been created, you can use hooks to programmatically display those widget areas throughout the theme. But if you're going to do that, it's very unlikely that you would want to use the plugin to create the widget areas. You might as well just create the widget areas programmatically too.
Not in the way you're probably thinking. The markup surrounding the widget area never changes. The only thing that changes is the dynamic content that displays within the pre-existing sidebar locations.