Exclude homepage posts from blog page

Hi everyone,
I have a problem with a newly created page, on my buddypress site i made a home.php and a blog.php.

the problem i have is that on my blog.php i only want to show my category id 45. (that i excluded from home.php with
<?php query_posts($query_string . ‘&cat=-45’); ?>

But my blog.php keeps showing my homepageblog that is created with category id 1. they still keep showing even when i put the code
<?php query_posts($query_string . ‘&cat=-1’); ?>
in blog.php

I hope someone knows what is wrong 🙁

Here is the code from my blog.php

[please follow http://codex.wordpress.org/Forum_Welcome#Posting_Code to mark posted code – some lines of the code below are broken]

<?php /* Template Name: Blog */ ?>

<?php get_header() ?> 

	<div id="content">

	<?php locate_template( array( 'leftsidebar.php' ), true ) ?>
		<div class="padder three">

		<?php do_action( 'bp_before_blog_home' ) ?>

		<?php do_action( 'template_notices' ) ?>

		<div class="page" id="blog-latest" role="main">

		<?php query_posts ($query_string . '&cat=45'); ?>

			<?php bp_dtheme_content_nav( 'nav-above' ); ?>

	<?php
	$temp = $wp_query;
	$wp_query= null;
	$wp_query = new WP_Query();
	$wp_query->query('posts_per_page=5'.'&paged='.$paged);
	while ($wp_query->have_posts()) : $wp_query->the_post();
	?>

					<?php do_action( 'bp_before_blog_post' ) ?>

					<div id="post-<?php the_ID(); ?>" <?php post_class(); ?>>

						<div class="author-box">
							<?php echo get_avatar( get_the_author_meta( 'user_email' ), '50' ); ?>
							<p><?php printf( _x( 'by %s', 'Post written by...', 'buddypress' ), bp_core_get_userlink( $post->post_author ) ) ?></p>

							<?php if ( is_sticky() ) : ?>
								<span class="activity sticky-post"><?php _ex( 'Featured', 'Sticky post', 'buddypress' ); ?></span>
							<?php endif; ?>
						</div>

						<div class="post-content">
							<h2 class="posttitle"><a>" rel="bookmark" title="<?php _e( 'Permanent Link to', 'buddypress' ) ?> <?php the_title_attribute(); ?>"><?php the_title(); ?></a></h2>

							<p class="date"><?php printf( __( '%1$s <span>in %2$s</span>', 'buddypress' ), get_the_date(), get_the_category_list( ', ' ) ); ?></p>

							<div class="entry">
								<?php the_content( __( 'Read the rest of this entry →', 'buddypress' ) ); ?>
								<?php wp_link_pages( array( 'before' => '<div class="page-link"><p>' . __( 'Pages: ', 'buddypress' ), 'after' => '</p></div>', 'next_or_number' => 'number' ) ); ?>
							</div>

							<p class="postmetadata"><?php the_tags( '<span class="tags">' . __( 'Tags: ', 'buddypress' ), ', ', '</span>' ); ?> <span class="comments"><?php comments_popup_link( __( 'No Comments »', 'buddypress' ), __( '1 Comment »', 'buddypress' ), __( '% Comments »', 'buddypress' ) ); ?></span></p>
						</div>

					</div>

					<?php do_action( 'bp_after_blog_post' ) ?>

				<?php endwhile; ?>

				<?php bp_dtheme_content_nav( 'nav-below' ); ?>

			<?php else : ?>

				<h2 class="center"><?php _e( 'Not Found', 'buddypress' ) ?></h2>
				<p class="center"><?php _e( 'Sorry, but you are looking for something that isn\'t here.', 'buddypress' ) ?></p>

				<?php get_search_form() ?>

			<?php endif; ?>
		</div>

		<?php do_action( 'bp_after_blog_home' ) ?>

		</div><!-- .padder -->
	</div><!-- #content -->

	<?php get_sidebar() ?>

<?php get_footer() ?>